Supervisor: Peter Peters
A native of Mauritius, I moved to France in 2006 to pursue my university studies. I currently hold a BSc. in Biochemistry and an MSc. in Structural and Functional Biochemistry from the University of Lyons (SE France). As part of my master’s, I have worked at the Institute of Biology and Chemistry of Proteins (Lyons, France) on the structural characterization of 5′-nucleotidase from human and from P. falciparum, mainly by X-ray crystallography and circular dichroism. After my graduation, I relocated to Singapore where I worked at the Duke-NUS Graduate Medical School for 4 months on the structural aspects of dengue-4 virus and neutralizing antibodies.
Little is known of the structure or localization of DISC1 multimers and complexes. As an Early Stage Researcher within work package 1 of the IN-SENS programme, I shall focus on the structural characterization of DISC1 multimers and complexes by single particle cryo-electron microscopy (cryo-EM) analysis and cryo-electron tomography. Furthermore, to investigate the correlation between DISC1 aggregate formation and the occurrence of disease, I shall use cryo-immunogold EM to track the formation of DISC1 aggregates and reveal the sub cellular localization of DISC1. This will be done in human neuroblastoma cells and brain tissue from model rats and human post-mortem brains.
DISC1 has a remarkably large number of protein interaction partners. So, I shall also examine the effect of DISC1 aggregate formation on DISC1 binding partners like NDEL1 and PCM1.